The exposure system was fed with a λ/8 monopole antenna (33 mm long) at the λ/4 position of the top plate of the cavity to excite only the fundamental TE102 mode, and it allowed for the uniform exposure of one 100-mm culture plate. The gas from an incubator was circulated throughout the chamber to maintain CO2 density and humidity. Additionally, cooling water was circulated through the bottom of the cavity to maintain the temperature at 37 ± 0.3°C.
The cells were exposed in a 100-mm Petri dish (I.D. 85 mm) in 18 ml of culture medium at 4 mm height.
For positive control, the cells were exposed to various doses (0.2-10 Gy) of 137Cs gamma radiation at a dose rate of 3.81 Gy/min.
Mess- und Berechnungsdetails
SAR inside the exposed medium was characterized by numerical simulations using the FDTD method and also by measurements using thermal probes located at nine points inside a Petri dish. When comparing the measurement data with the simulation results, the inevitable modelling errors seem to be rather big, but they show a similar trend for each position. The reported SARs correspond to the mean of the values measured (?) at three points along a radial path from the center to the edge of the dish.
Chauhan V et al.
Evaluating the biological effects of intermittent 1.9 GHz pulse-modulated radiofrequency fields in a series of human-derived cell lines.
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Effects of continuous and intermittent exposure to RF fields with a wide range of SARs on cell growth, survival, and cell cycle distribution.
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