The system was allowed to equilibrate for 1 h prior to energizing the waveguides. The temperature within the cell cultures was monitored every 60 s and maintained within 37.0 ± 0.5°C for the sham, negative (incubator) control and RF-exposed samples. The samples exposed at an SAR of 10 W/kg showed cyclical fluctuations of ±0.2°C.
The 60-mm dishes containing the cell cultures in 10 ml of medium were placed atop a series of circularly polarized cylindrical waveguide applicators.
Concurrent negative and positive controls were included in each experiment. The positive (heat-shock) controls were placed on a heating block within an incubator and were maintained at 43°C for 1 h. The negative controls were placed within the same incubator but were not subjected to the heat shock.
The SAR distribution at the plane of the cells was estimated to be ±24% of the mean SAR for each RF-field treatment group, while the maximum to minimum SAR ratio within the sample region was approximately 4:1 [Gajda et al., 2002].
Gajda GB et al.
Cylindrical waveguide applicator for in vitro exposure of cell culture samples to 1.9-GHz radiofrequency fields
Kayhan H et al.
Does MW Radiation Affect Gene Expression, Apoptotic Level, and Cell Cycle Progression of Human SH-SY5Y Neuroblastoma Cells?
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Differential Pro-Inflammatory Responses of Astrocytes and Microglia Involve STAT3 Activation in Response to 1800 MHz Radiofrequency Fields
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Acute radio frequency irradiation does not affect cell cycle, cellular migration, and invasion
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Comparative study of cell cycle kinetics and induction of apoptosis or necrosis after exposure of human mono mac 6 cells to radiofrequency radiation
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Effects of in vivo exposure to GSM-modulated 900 MHz radiation on mouse peripheral lymphocytes
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Immunotropic effects in cultured human blood mononuclear cells pre-exposed to low-level 1300 MHz pulse-modulated microwave field