研究のタイプ: 医学／生物学の研究 (experimental study)

[思春期の雄ラット心臓の形態および生化学に対する900MHz電磁界への長期ばく露の影響] med./bio.

Effects of long-term exposure to 900 megahertz electromagnetic field on heart morphology and biochemistry of male adolescent rats

著者: Kerimoglu G, Mercantepe T, Erol HS, Turgut A, Kaya H, Colakoglu S, Odaci E
掲載誌: Biotech Histochem 2016; 91 (7): 445-454
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この研究は、21日齢雄ラット（n=24）を同数ずつの3群（無処置対照群、擬似ばく露群、ばく露群）に分け、ばく露群は、Plexiglas製のばく露ケージに入れて1日1時間の900MHzばく露を生後21日目から59日目まで継続した。生後60日目に全てのラットを屠殺して心臓を摘出し、顕微鏡的および生化学的検査を行った。その結果、対照群に比べばく露群では、マロンジアルデヒドおよびスーパーオキシドジスムターゼのレベル上昇、グルタチオンおよびカタラーゼのレベル低下が見られた；ばく露群では、心筋の構造的変化と毛細管鬱血が観察された､等々の所見を報告している。

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研究目的（著者による）

The effects of exposure of adolescent rats to a 900 MHz electromagnetic field on morphology and oxidative stress in the heart should be investigated.

詳細情報

Male 21-day-old rats were divided into 3 groups (n=8 each): 1) exposure to the electromagnetic field, 2) sham exposure and 3) cage control. All rats were killed on postnatal day 60 and hearts were investigated.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
ばく露時間: 1 h/day between postnatal days 21 and 59
  • 電力: 300 mW (approximate output power)
  • 電界強度: 7 V/m mean (on the outside the empty plexiglass cage floor)
  • 電界強度: 9.54 V/m mean (on the inside the empty plexiglass cage floor)
  • 電界強度: 5.4 V/m mean (with rats in cage on the outside the plexiglass cage floor)
  • 電界強度: 8.4 V/m mean (with rats in cage on the inside the plexiglass cage floor; rat exposure value)
  • 電力密度: 0.187 W/m² mean (rat exposure value)
  • SAR: 0.0093 W/kg

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 1 h/day between postnatal days 21 and 59
ばく露装置
ばく露の発生源／構造
チャンバの詳細 plexiglass cage (42 cm length x 30 cm width x 50 cm height)
ばく露装置の詳細 half-wave dipole antenna was made of 1 mm wide x 15 cm long copper plate and was inserted into the central area of the plexiglass cage, approximately 11 cm inside the opening; rats could move freely during exposure; temperature during exposure was 21.43°C ± 1.07°C and humidity was 59.8% ± 4.11%
Sham exposure A sham exposure was conducted.
Additional information all groups were kept in the same room in separate cages under the same environmental conditions, except during sham and EMF exposure; temperature during sham exposure was 21.30°C ± 1.08°C and humidity was 59.7% ± 4.34%
パラメータ
測定量 値 種別 Method Mass 備考
電力 300 mW - - - approximate output power
電界強度 7 V/m mean 測定値 - on the outside the empty plexiglass cage floor
電界強度 9.54 V/m mean 測定値 - on the inside the empty plexiglass cage floor
電界強度 5.4 V/m mean 測定値 - with rats in cage on the outside the plexiglass cage floor
電界強度 8.4 V/m mean 測定値 - with rats in cage on the inside the plexiglass cage floor; rat exposure value
電力密度 0.187 W/m² mean - - rat exposure value
SAR 0.0093 W/kg - 計算値 whole body -
Additional parameter details

electric field strengths were measured at nine points on the inside and outside of the plexiglass cage floor

Reference articles

  • Odaci E et al. (2015): [30日間にわたる1日1時間の900MHz電磁界へのばく露によるラット精巣の組織病理および生化学的変化はない]
  • Turedi S et al. (2015): [900MHz電磁界への出生前ばく露の21日齢雄ラットの心臓への影響]
  • Topal Z et al. (2015): [21日齢の新生仔雄ラットの肝臓に対する900 MHz電磁界への出生前の長時間ばく露の影響]
  • Hanci H et al. (2013): [21日齢のラットの精巣に対する900 MHz電磁界への出生前ばく露の影響]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Apoptosis and lipid peroxidation were significantly increased in the exposure group (group 1) compared to the sham exposure group (group 2). Additionally, in comparison to the cage control (group 3), the superoxide dismutase enzyme activity was significantly increased and the enzyme activity of catalase and the amount of glutathione were significantly decreased in the exposure group. However, in the sham exposure group, apoptosis and all oxidative stress parameters except lipid peroxidation were significantly different compared to the cage control as well.
In histology and ultrastructure, the sham exposure and cage control showed no histopathological changes, while the exposure group exhibited structural changes and capillary congestion in the myocardium, decreased myofilaments and pronounced vacuolization.
There were no significant differences in body or heart weights between the groups.
The authors conclude that exposure of adolescent rats to a 900 MHz electromagnetic field might induce oxidative stress causing morphological alterations in the heart.

研究の種別:

研究助成

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