研究のタイプ: 医学／生物学の研究 (experimental study)

[携帯電話電磁界による肝臓のフリーラジカル損傷は抗酸化剤N-アセチルシステインおよびエピガロカテキンガレートにより抑制される] med./bio.

Mobile phone radiation-induced free radical damage in the liver is inhibited by the antioxidants N-acetyl cysteine and epigallocatechin-gallate

著者: Ozgur E, Güler G, Seyhan N
掲載誌: Int J Radiat Biol 2010; 86 (11): 935-945
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この研究は、モルモットの肝臓に対する携帯電話様無線周波電磁界(RFR)の影響を酸化損傷および抗酸化酵素の状態を指標に調べると共に、N-アセチルシステイン(NAC)およびエピガロカテキンガレート(EGGG)による酸化損傷の防護作用を調べた。モルモットは9群に分けた：3通りのばく露条件（擬似ばく露・10分間RFRばく露・20分間RFRばく露）×3通りの抗酸化剤条件（生食・NAC・EGCG）である。擬似ばく露は10分間と20分間をそれぞれn=6行い、その他の群はn=6、生食や抗酸化剤は腹腔内注射した。GSM1800MHz変調信号(全身平均SAR0.38W/kg)に1日10または20分間、7日間)ばく露した。その結果、RFRばく露後のモルモット肝臓で、マロンジアルデヒド(MDA)および全酸化窒素(NOx)の有意なレベル上昇、スーパーオキシドジスムターゼ(SOD)、ミエロペルオキシダーゼ、グルタチオン・ペルオキシダーゼ(GSH-Px)の活性の有意な減少が見られた；NAC投与群でのみ肝臓GSH-Px活性上昇が見られ、EGCG投与群でのみMDAレベル低下が見られた；酸化損傷の程度はばく露時間に比例した、と報告している。

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研究目的（著者による）

To study oxidative damage and antioxidant enzyme status in the liver of guinea pigs exposed to mobile phone-like radiofrequency irradiation and the potential protective effects of N-acetyl-cysteine and epigallocatechin-gallate (component of green tea) on the oxidative damage.

詳細情報

96 male guinea pigs were divided into nine groups. Twelve animals were included in each of the three sham exposed groups (treatment with saline solution, N-acetyl-cysteine or epigallocatechin-gallate, groups 1-3). In each of these groups, six animals were subjected to 10 minutes sham exposure and the other six for 20 minutes sham exposure. Ten animals were used in each of the other six treatment groups: 4.) 10 minutes radiofrequency exposure + 1 ml saline solution, 5.) 20 minutes radiofrequency exposure + 1 ml saline solution, 6.) 10 minutes radiofrequency exposure + 1 ml N-acetyl-cysteine (300 mg/kg body weight), 7.) 20 minutes radiofrequency exposure + 1 ml N-acetyl-cysteine, 8.) 10 minutes radiofrequency exposure + 1 ml epigallocatechin-gallate (12.5 mg/kg body weight), 9.) 20 minutes radiofrequency exposure + 1 ml epigallocatechin-gallate. The saline or antioxidant solutions were injected 30 minutes before exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
Modulation type: pulsed
ばく露時間: continuous for 10 min./day on 7 days or 20 min./day on 7 days
  • SAR: 0.38 W/kg average over mass (whole body)

General information

rats were divided into the following groups: i) sham exposure + 1 ml saline ii) sham exposure + 1 ml N-acetyl cysteine (NAC) iii) sham exposure + 1 ml epigallocatechin-gallate (EGCG) iv) 10 min. RF exposure + 1 ml saline v) 20 min. RF exposure + 1 ml saline vi) 10 min. RF exposure + 1 ml NAC vii) 20 min. RF exposure + 1 ml NAC viii) 10 min. RF exposure + 1 ml EGCG ix) 20 min. RF exposure + 1 ml EGCG

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 10 min./day on 7 days or 20 min./day on 7 days
Modulation
Modulation type pulsed
Pulse width 0.576 ms
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
ばく露装置
ばく露の発生源／構造
チャンバの詳細 shielded room
ばく露装置の詳細 guinea pigs kept in 8 cm x 10 cm x 18 cm individual Plexiglas cages during exposure
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 0.38 W/kg average over mass 推定値 whole body -

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
  • liver
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Significant increases in malondialdehyde and total nitric oxide levels and decreases in enzyme activities of superoxide dismutase, myeloperoxidase and glutathione peroxidase were found in the liver of guinea pigs after radiofrequency exposure (in all exposed groups). The extent of oxidative damage was proportional to the duration of exposure. Treatment with N-acetyl-cysteine and epigallocatechin-gallate markedly decreased lipid peroxidation and nitrite oxide levels and affected the antioxidant status in animals exposed to mobile phone irradiation.
The authors conclude that mobile phone-like irradiation induces oxidative damage and changes the enzyme activities of antioxidant enzymes in the liver. The adverse effect of radiofrequency exposure may be related to the duration of mobile phone use. N-acetyl-cysteine and epigallocatechin-gallate protect the liver tissue against the radiofrequency-induced oxidative damage and enhance antioxidant enzyme activities.

研究の種別:

研究助成

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