研究のタイプ: 医学/生物学の研究 (experimental study)

[ワイヤレス(2.45 GHz)機器によって生じたラットの睾丸における酸化損傷に対するメラトニンの防護作用] med./bio.

Protective effects of melatonin against oxidative injury in rat testis induced by wireless (2.45 GHz) devices.

掲載誌: Andrologia 2014; 46 (1): 65-72

この研究は、2.45GHzの電磁放射(EMR)による酸化ストレスに依存した睾丸の損傷に対するメラトニンの防護作用の可能性を調べた動物実験である。32匹の雄のWistarアルビノラットを、ケージ対照群(A1)、シャム対照群(A2)、2.45GHz ばく露群(B)、2.45GHzばく露メラトニン投与群(C)に四等分した。B及びC群は2.45GHzのEMR(放射状に配置した6本のチューブ内で頭部を中心に向けてラット固定、中心に配置したモノポールアンテナからEMR照射アンテナから睾丸までの距離は約1m、電力密度1mW/m2、全身平均比吸収率SAR)は0.008-4.2W/kgの範囲)に60分/日、30日間ばく露した。その結果、B群の脂質の過酸化レベルはA1及びA2群よりも高かった;メラトニン投与はEMRによる脂質の過酸化の上昇を防いだ;D群【訳注:C群の間違いであろう】のグルタチオンGSH)及びグルタチオンペロキシダーゼ(GSH-Px)のレベルはばく露群よりも高かった;ビタミンA及びE濃度はばく露群で低下していた;メラトニンビタミンEのレベル低下を防いだ、と報告している。

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研究目的(著者による)

To investigate the effects of exposure to a 2.45 GHz electromagnetic field on oxidative stress in rat testis, as well as the possible protective effects of melatonin.

詳細情報

Rats were divided into four groups (n=8 per group): 1.) cage control, 2.) sham exposure + saline, 3.) exposure and 4.) exposure + melatonin (10 mg/kg per day). After 30 days of exposure, rats were sacrificed and testis were taken.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2.45 GHz
Modulation type: pulsed
ばく露時間: continuous for 1 h/day on 30 days

General information

Rats were treated in the following four groups: i) cage control ii) sham exposure iii) EMF exposure iv) EMF exposure + 10 mg/kg per day melatonin administration

ばく露1

主たる特性
周波数 2.45 GHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 h/day on 30 days
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源/構造
  • half-wave dipole or monopole (varying indications within the text; remark EMF-Portal)
Distance between exposed object and exposure source 1 m
ばく露装置の詳細 15 cm long cylindrical constrainers with a diameter of 5 cm, holding one rat each, positioned radially on a plexiglass ground plate with the antenna in the center; 8 rats (Figure shows 6 rats (?)) placed with their noses near the antenna (distance between testis of the rat and antenna nearly 1 m (?!); exposure system kept in a Faraday cage
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電界強度 11 V/m - 測定値 - -
電力密度 1 mW/m² - - - -
SAR 0.1 W/kg - 計算値 whole body -

Reference articles

  • Gumral N et al. (2009): [無線装置からの2.45GHz放射によるラット血液中の酸化ストレスに対するセレンおよびL-カルニチンの効果]
  • Naziroglu M et al. (2009): [ラットの脳内における無線機器(2.45 GHz)由来の酸化ストレス及び脳電図記録にL-カルニチン及びセレンが及ぼす変調作用]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

No significant difference in the weight of the testis was found among the groups.
The values for lipid peroxidation in the exposed group were significantly higher than in the cage control and the sham exposure group. However, exposed rats treated with melatonin had significant lower values for lipid peroxidation when compared to the exposed group without melatonin treatment.
The level of glutathione was significantly decreased in the exposed group with melatonin compared to the exposed group without melatonin administration. The enzyme activity of the glutathione peroxidase was significantly increased in the exposed group with melatonin in comparison to the exposed animals without melatonin and the control groups.
A significant decrease in the levels of vitamin A and a significant increase in the vitamin E levels were found in both exposure groups when compared to the control groups. Moreover, the level of vitamin E in the exposure group with melatonin was significantly higher than in the exposure group without melatonin.
Among the groups, no difference in the content of vitamin C and beta-carotene was found.
The authors conclude that exposure to an electromagnetic field could induce oxidative damage in rat testis. A melatonin administration could prevent oxidative damage and support the antioxidant defense system.

研究の種別:

研究助成

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