この研究は、2.45GHzの電磁放射（EMR）による酸化ストレスに依存した睾丸の損傷に対するメラトニンの防護作用の可能性を調べた動物実験である。32匹の雄のWistarアルビノラットを、ケージ対照群（A1）、シャム対照群（A2）、2.45GHz ばく露群（B）、2.45GHzばく露＋メラトニン投与群（C）に四等分した。B及びC群は2.45GHzのEMR（放射状に配置した6本のチューブ内で頭部を中心に向けてラットを固定、中心に配置したモノポールアンテナからEMR照射、アンテナから睾丸までの距離は約1m、電力密度1mW/m2、全身平均の比吸収率（SAR）は0.008-4.2W/kgの範囲）に60分／日、30日間ばく露した。その結果、B群の脂質の過酸化レベルはA1及びA2群よりも高かった；メラトニン投与はEMRによる脂質の過酸化の上昇を防いだ；D群【訳注：C群の間違いであろう】のグルタチオン（GSH）及びグルタチオンペロキシダーゼ（GSH-Px）のレベルはばく露群よりも高かった；ビタミンA及びE濃度はばく露群で低下していた；メラトニンはビタミンEのレベル低下を防いだ、と報告している。
The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →
To investigate the effects of exposure to a 2.45 GHz electromagnetic field on oxidative stress in rat testis, as well as the possible protective effects of melatonin.
Rats were divided into four groups (n=8 per group): 1.) cage control, 2.) sham exposure + saline, 3.) exposure and 4.) exposure + melatonin (10 mg/kg per day). After 30 days of exposure, rats were sacrificed and testis were taken.
Rats were treated in the following four groups: i) cage control ii) sham exposure iii) EMF exposure iv) EMF exposure + 10 mg/kg per day melatonin administration
| 周波数 | 2.45 GHz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 1 h/day on 30 days |
| Modulation type | pulsed |
|---|---|
| Repetition frequency | 217 Hz |
| ばく露の発生源／構造 | |
|---|---|
| Distance between exposed object and exposure source | 1 m |
| ばく露装置の詳細 | 15 cm long cylindrical constrainers with a diameter of 5 cm, holding one rat each, positioned radially on a plexiglass ground plate with the antenna in the center; 8 rats (Figure shows 6 rats (?)) placed with their noses near the antenna (distance between testis of the rat and antenna nearly 1 m (?!); exposure system kept in a Faraday cage |
| Sham exposure | A sham exposure was conducted. |
No significant difference in the weight of the testis was found among the groups.
The values for lipid peroxidation in the exposed group were significantly higher than in the cage control and the sham exposure group. However, exposed rats treated with melatonin had significant lower values for lipid peroxidation when compared to the exposed group without melatonin treatment.
The level of glutathione was significantly decreased in the exposed group with melatonin compared to the exposed group without melatonin administration. The enzyme activity of the glutathione peroxidase was significantly increased in the exposed group with melatonin in comparison to the exposed animals without melatonin and the control groups.
A significant decrease in the levels of vitamin A and a significant increase in the vitamin E levels were found in both exposure groups when compared to the control groups. Moreover, the level of vitamin E in the exposure group with melatonin was significantly higher than in the exposure group without melatonin.
Among the groups, no difference in the content of vitamin C and beta-carotene was found.
The authors conclude that exposure to an electromagnetic field could induce oxidative damage in rat testis. A melatonin administration could prevent oxidative damage and support the antioxidant defense system.
このウェブサイトはクッキー（Cookies）を使って、最善のブラウジングエクスペリエンスを提供しています。あなたがこのウェブサイトを継続して使用することで、私たちがクッキーを使用することを許可することになります。
