研究のタイプ: 医学/生物学の研究 (experimental study)

[新しい抗酸化剤であるカフェイン酸フェネチルエステルはラットでの長期携帯電話ばく露誘発腎障害を防止する:マロンジアルデヒド、N-アセチル-β-D-グルコスアミニダーゼおよび酸化窒素の測定の予後値] med./bio.

A novel antioxidant agent caffeic acid phenethyl ester prevents long-term mobile phone exposure-induced renal impairment in rat. Prognostic value of malondialdehyde, N-acetyl-beta-D-glucosaminidase and nitric oxide determination.

掲載誌: Mol Cell Biochem 2005; 277 (1-2): 73-80

この研究は、900 MHz携帯電話電磁放射(EMR)への長期ばく露ラットに与え、酸化ストレス誘導するか否か、さらに、カフェ酸フェネチルエステル(CAPE:フラボノイド様物質)がEMR誘導性の腎障害から腎組織を保護する役割を持つか否かを調べた。スーパーオキシド一酸化窒素NO)などのROS(酸化ストレスによる腎障害誘発の指標)、マロンジアルデヒドMDA脂質過酸化の指標)、尿中N-アセチル-ベータ-D-グルコサミニダーゼ(NAG、腎尿細管損傷マーカー)のレベルを測定した。腎組織スーパーオキシドジスムターゼ(SOD)、カタラーゼ(CAT)、およびグルタチオンペルオキシダーゼGSH-Px)の活性を測定し、抗酸化状態の変化を評価した。実験ではラットを次の4群に分けた(各n = 10): i)対照群ストレスおよびEMRなし)、ii)擬似ばく露群、iii)900MHz EMRばく露群、iv)900 MHz EMRばく露+ CAPE処置(EMR + CAPE群)。 その結果、EMRばく露群では、腎組織のMDA、NOレベルおよび尿中NAGレベルが有意に上昇し、腎組織のSOD、CAT、およびGSH-Pxの活性は低下した;CAPE処置はこれらの影響を有意に逆転させた、と報告している。

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研究目的(著者による)

To study long-term applied 900 MHz emitting mobile phone-induced oxidative stress that promotes production of reactive oxygen species (ROS) and to investigate the role of CAPE (caffeic acid phenethyl ester) on kidney tissue against the possible electromagnetic irradiation-induced renal impairment in rats.

詳細情報

Caffeic acid phenethyl ester (CAPE) is one of the major components of honeybee propolis. It has been used in folk medicine for many years in Middle East countries. It was found to be a potent free radical scavenger and antioxidant.
ROS such as superoxide and nitric oxide may contribute to the pathophysiology of electromagnetic irradiation-induced renal impairment.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: repeated daily exposure, 30 min/day for 3 months
  • 電力: 2 W peak
  • 電力密度: 1.04 mW/cm² unspecified
  • SAR: 0.016 W/kg average over mass (whole body)
  • SAR: 4 W/kg maximum (unspecified)

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 repeated daily exposure, 30 min/day for 3 months
Modulation
Modulation type pulsed
ばく露装置
ばく露の発生源/構造
チャンバの詳細 A special exposure device with six exposure antennas was used. One exposure system consisted of a ventilated plastic tube cage (12 cm long x 5 cm in diameter) and a half-wave dipole antenna.
ばく露装置の詳細 The whole body of the rat was positioned in close contact above the antenna.
Additional information Rats were allocated to four groups: control (without stress and EMR), sham exposed, EMR exposed, and EMR exposed + CAPE treated. CAPE was injected i.p. daily immediately before exposure. Sham exposed rats received isotonic saline solution instead.
パラメータ
測定量 種別 Method Mass 備考
電力 2 W peak - - -
電力密度 1.04 mW/cm² unspecified 測定値 - -
SAR 0.016 W/kg average over mass 測定値 whole body -
SAR 4 W/kg maximum 測定値 unspecified -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
  • kidney
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In the electromagnetic field exposed group, while tissue malondialdehyde, nitric oxide levels and urinary NAG levels increased, the activities of superoxide dismutase, catalase, and glutathione peroxidase in renal tissue were reduced. CAPE treatment reversed these effects as well.
In conclusion, the data demonstrate the role of oxidative mechanisms in 900 MHz mobile phone-induced renal tissue damage. CAPE, via its free radical scavenging and antioxidant properties, ameliorates oxidative renal damage. These data strongly suggest that CAPE exhibits a protective effect on mobile phone-induced and free radical mediated oxidative renal impairment in rats.

研究の種別:

研究助成

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