研究のタイプ: 医学/生物学の研究 (experimental study)

[雄のラットの生殖パターンに対する携帯電話放射ばく露の影響の証拠:活性酸素種の役割] med./bio.

Evidence for mobile phone radiation exposure effects on reproductive pattern of male rats: role of ROS.

掲載誌: Electromagn Biol Med 2012; 31 (3): 213-222

この研究は、雄のラット生殖パターンに対する携帯電話放射ばく露の影響を、精子生化学的指標により調べた。70日齢のWister系雄ラットばく露群(6匹;900MHzGSM携帯電話放射を1日2時間、45日間)、擬似ばく露群(6匹)で比較した。固定用プレキシガラス製ケージにラットを入れ、大きなケージ内に置いた(大きなケージ上面に携帯電話を置き、その下に固定用ケージを置いた)。その結果、ばく露群でテストステロンレベルの有意な低下とカスパーゼ3活性の上昇が見られた;透過型電子顕微鏡検査により、ばく露群で精子ミトコンドリア鞘の頭部と中間部の変形が観察されたことを報告し、活性酸素種の影響を考察している。また、別の実験として、雌雄別々に上記の方法で45日間のばく露擬似ばく露)を終了した後、ばく露した雄3匹と雌6匹の組、擬似ばく露した雄3匹と雌6匹の組に生殖をさせ、子孫を調べた結果、擬似ばく露組の子孫に比べ、ばく露組の子孫の方が数および体重が低下したことも報告している。

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研究目的(著者による)

To examine whether a GSM signal effects the reproductive pattern by targeting the biochemistry of sperm.

詳細情報

Two experiments were performed. For the first experiment, male rats were divided into a sham exposure or an exposure group (n=6 per group). Blood was collected immediately after exposure for a testosterone assay. Immediately after the last exposure, the rats were sacrificed and the sperms collected. The caspase-3 activity was measured and sperm samples were morphological examined.
For the second experiment, 18 rats were divided into two groups: sham exposed group (male=3, female =6) and exposed group (male=3, female=6). After the exposure, rats of each group were mated. The number and the weight of the pups were regarded.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 2 h/day for 45 days
  • 電力: 2 mW maximum
  • SAR: 0.9 W/kg maximum (manufacturer's data)

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 h/day for 45 days
Modulation
Modulation type pulsed
Pulse width 0.577 ms
Repetition frequency 216.7 Hz
Additional information

GSM mode

ばく露装置
ばく露の発生源/構造
  • セルラ-電話
チャンバの詳細 rats placed individually in 18-22 cm long, 5.7 cm high and 4-7 cm wide Plexiglas cages, fixed with anechoic material and ventilated with 1 cm diameter holes; the cages were pasted with anechoic material on two sides of each box (top was not covered)
ばく露装置の詳細 mobile phone positioned on the top of the cage; mobility of the rats in the cage was limited to 1-2 cm; cell phones in silent mode without vibration
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力 2 mW maximum 測定値 - -
SAR 0.9 W/kg maximum - - manufacturer's data

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Spermatozoa from exposed rats showed significant morphological changes in midpiece region, microtubules, outer dense fibers of mitochondria and membranes and distortions in sperm head, compared to sham exposed rats. A statistically significant increase in activation of caspase-3 was observed in the sperm of exposed animals compared to sham exposed animals. Serum testosterone level was significantly decreased in exposed animals in comparison to sham exposed ones.
Exposed parents produced litters with a significantly decreased number of pups and the average body weight of the pups was significantly lower in comparison to those of sham exposed parents.

研究の種別:

研究助成

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