研究のタイプ: 医学/生物学の研究 (experimental study)

[スイスアルビノマウスでの無線周波放射(900 MHz)による精巣生殖細胞でのDNA損傷および細胞周期停止] med./bio.

Radiofrequency radiation (900 MHz)-induced DNA damage and cell cycle arrest in testicular germ cells in swiss albino mice.

掲載誌: Toxicol Ind Health 2017; 33 (4): 373-384

この研究は、スイスアルビノマウスに無線周波放射(RFR:900 MHz、1日のばく露時間は4時間または8時間、35日間継続)を与え、1群はばく露終了直後、他群はばく露終了からさらに35日間の回復期間を経た後に、精巣生殖細胞の変化を調べた。その結果、RFRばく露群にミトコンドリア膜電位の低下が見られた;生殖細胞の損傷指標上昇、精子頭部異常細胞周期異常なども見られた;しかしこれらの変化は、ばく露後の35日間で回復が見られた、と報告している。

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研究目的(著者による)

The effects of exposure of mice to a 900 MHz electromagnetic field (GSM) on the cell cycle and DNA damage in sperms and testes histology should be investigated.

詳細情報

Mice were divided into 5 groups (n=15 each): exposure to the electromagnetic field for 1) 4 h/day for 35 days, 2) 4 h/day for 35 days and subsequent unexposed maintenance for 35 days, 3) 8 h/day for 35 days, 4) 8 h/day for 35 days and subsequent unexposed maintenance for 35 days, 5) control group. Groups 2 and 4 were used to analyze post-exposure recovery effects.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 902.4 MHz
Modulation type: pulsed
ばく露時間: 4 h/day for 35 days
  • 電力密度: 2.717 W/m² maximum (at minimum distance of animals to antenna (4.5 cm))
  • 電力密度: 0.284 W/m² minimum (at maximum distance of animals to antenna (22.5 cm))
  • SAR: 0.0516 W/kg maximum
  • SAR: 0.0054 W/kg minimum
  • 電力: 250 mW mean
ばく露2: 902.4 MHz
Modulation type: pulsed
ばく露時間: 8 h/day for 35 days
  • 電力密度: 2.717 W/m² maximum (at minimum distance of animals to antenna (4.5 cm))
  • 電力密度: 0.284 W/m² minimum (at maximum distance of animals to antenna (22.5 cm))
  • SAR: 0.0516 W/kg maximum
  • SAR: 0.0054 W/kg minimum
  • 電力: 250 mW mean

ばく露1

主たる特性
周波数 902.4 MHz
タイプ
  • electromagnetic field
ばく露時間 4 h/day for 35 days
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

similar to GSM

ばく露装置
ばく露の発生源/構造
  • antenna
チャンバの詳細 chromium-nickel metal container
ばく露装置の詳細 animals could move freely during exposure; minimum and maximum distance of animals from antenna was 4.5 cm and 22.5 cm
パラメータ
測定量 種別 Method Mass 備考
電力密度 2.717 W/m² maximum 測定値 - at minimum distance of animals to antenna (4.5 cm)
電力密度 0.284 W/m² minimum 測定値 - at maximum distance of animals to antenna (22.5 cm)
SAR 0.0516 W/kg maximum - - -
SAR 0.0054 W/kg minimum - - -
電力 250 mW mean 測定値 - -

ばく露2

主たる特性
周波数 902.4 MHz
タイプ
  • electromagnetic field
ばく露時間 8 h/day for 35 days
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

similar to GSM

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
電力密度 2.717 W/m² maximum 測定値 - at minimum distance of animals to antenna (4.5 cm)
電力密度 0.284 W/m² minimum 測定値 - at maximum distance of animals to antenna (22.5 cm)
SAR 0.0516 W/kg maximum - - -
SAR 0.0054 W/kg minimum - - -
電力 250 mW mean 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Exposure to the electromagnetic field of groups 3 and 4 caused a significantly reduced mitochondrial membrane potential compared to the control group, though potential was higher after recovery (group 4). Depolarization of the mitochondrial membrane leads to impaired cellular redox homeostasis causing oxidative stress.
The number of abnormal sperm cells with head defects was significantly increased in exposure groups 1, 3 and 4 compared to the control group.
Analysis of germ cell subpopulations in testis showed a significant increase of spermatogonia and a significant decrease in spermatids in groups 3 and 4 compared to the control group. This indicated cell cycle arrest in the pre-meiotic stage of spermatogenesis, which resulted in loss of post-meiotic germ cells.
Histological investigation showed significantly reduced epithelial area and significantly increased loss of tissue in seminiferous tubules in group 3 compared to the control group, but not in group 4. DNA damage was significantly increased in exposure groups 1, 3 and 4 compared to the control group, with most damage found in groups 1 and 3.
The authors conclude that exposure of mice to a 900 MHz electromagnetic field (GSM) might induce oxidative stress, which causes DNA damage that leads to alterations of the cell cycle and to testicular damage. However, these effects might be reversible.

研究の種別:

研究助成

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