研究のタイプ: 医学/生物学の研究 (experimental study)

[900MHz電磁界にばく露したラットの脳の異なる領域における酸化ストレスおよび抗酸化防御の評価] med./bio.

Evaluation of oxidant stress and antioxidant defense in discrete brain regions of rats exposed to 900 MHz radiation.

掲載誌: Bratisl Lek Listy 2014; 115 (5): 260-266

この研究は、ウィスター雄ラット(6-8週齢:n=36)を用いて、脳の異なる領域におけるチオバルビツール酸反応物質(TBARS)、総抗酸化能(TA)、グルタチオンSトランスフェラーゼ(GST)のレベルに対する900MHz無線周波電磁界RF-EMR)の影響を調べた。ラットは12匹ずつの3群(ケージ対照群、擬似ばく露群、ばく露群)とし、ばく露群は900MHzRF-EMR(GSM携帯電話のサイレントモードを使用:ピーク電力密度146.60 µW/cm2、1日1時間)に4週間ばく露した。実験29日目に行動学的分析を行い、その後各群6匹について、扁桃、海馬前頭葉小脳組織切片を検査した。その結果、ばく露群で行動学的タスクの成績の変化が見られた;全ての部位でTBARSレベル上昇が見られた;海馬のみでGSTレベルが低下した、などを報告している。

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研究目的(著者による)

The effects of a whole body exposure of adolescent rats to a 900 MHz electromagnetic GSM field on oxidative stress and the antioxidative system in discrete brain sections should be investigated.

詳細情報

36 rats were equally separated into the three following groups (n=12 in each group): 1) cage control, 2) sham exposure and 3) exposure. Subsequent to exposure (day 29), behavioral analysis was conducted (not part of the present paper, published here: Narayanan et al. 2013) and afterwards, six animals of each group were sacrificed and examined.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 890–915 MHz
Modulation type: pulsed
ばく露時間: intermittent for 1 h/day for 28 days
  • SAR: 1.58 W/kg (according to manufacturer)
  • 電力密度: 146.6 µW/cm² peak (3 cm away from the phone during incoming call)
  • 電力密度: 2.109 nW/cm² peak (3 cm away from the phone in "ON" mode during pause intervals)

ばく露1

主たる特性
周波数 890–915 MHz
タイプ
  • electromagnetic field
ばく露時間 intermittent for 1 h/day for 28 days
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源/構造
  • セルラ-電話
チャンバの詳細 polypropylene cages (3 animals per cage) measuring 41 cm Œ 28 cm Œ 14 cm, temperature 23°C ± 2°C
ばく露装置の詳細 phone in silence mode was placed in a wood bottom bamboo wire mesh cage (12 cm Œ 7 cm Œ 7 cm) in the center of the home cage with 3 rats; daily exposure composed of 50 unattended calls within 1 hour, each lasting for 45 seconds, followed by a 15-second pause interval before next call
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 1.58 W/kg - - - according to manufacturer
電力密度 146.6 µW/cm² peak 測定値 - 3 cm away from the phone during incoming call
電力密度 2.109 nW/cm² peak 測定値 - 3 cm away from the phone in "ON" mode during pause intervals

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
  • 脳/腫瘍
  • amygdala, hippocampus, frontal cerebral cortex, cerebellum
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The level of thiobarbituric acid reactive substances was significantly increased in samples of exposed animals compared to sham exposure and cage control in all investigated brain regions. Moreover, exposure samples showed a significant decrease in the total antioxidative activity in the amygdala and cerebellum but it was not significantly changed in other brain regions. Glutathione S-transferase enzyme activity was significantly decreased in the hippocampus in the exposure group compared to sham exposure and cage control, but it was unaltered in other brain regions studied.
The authors conclude that an exposure of rats to a 900 MHz electromagnetic GSM field causes oxidative stress with regional differences in rat brain, which might be related to behavioral changes observed in Narayanan et al. 2013.

研究の種別:

研究助成

関連論文