Acute exposure to low-level CW and GSM-modulated 900 MHz radiofrequency does not affect Ba(2+) currents through voltage-gated calcium channels in rat cortical neurons.
[Akute Exposition mit schwachen kontinuierliche Welle- und GSM-modulierten 900 MHz-Hochfrequenz-Feldern beeinflusst nicht die Ba(2+)- Ströme durch spannungsabhängige Calciumkanäle in kortikalen Neuronen der Ratte].
Platano D, Mesirca P, Paffi A, Pellegrino M, Liberti M, Apollonio F, Bersani F, Aicardi G
The exposure system, especially designed for electrophysiological patch-clamp recording, has been described in [Liberti et al., 2004]. An open propagating structure consisting of a Co-Planar Waveguide (22.0 cm x 10.2 cm x 0.4 cm) was positioned on the stage of a microscope. The upper side of the dielectric substrate (glass) was plated with three thin conductive gold layers leaving two windows for the visualization of the cell culture.
A 35-mm Petri dish was held in the center of the exposure system by a 3-mm polystyrene mask.
The structure propagated only the fundamental mode (quasi-transverse electromagnetic mode) in which the direction of the EF was almost parallel to the Petri dish surface and orthogonal to the glass microelectrode, thus minimising interference. The system assured a good homogeneity of the field applied to the sample.
Frequency, intensity and duration of the field were guaranteed by a computer-assisted generator. Before and after each experiment, input and reflected powers were measured to evaluate the correct working of the exposure system.
Marchionni I et al.
Comparison between low-level 50 Hz and 900 MHz electromagnetic stimulation on single channel ionic currents and on firing frequency in dorsal root ganglion isolated neurons.
Green AC et al.
An investigation of the effects of TETRA RF fields on intracellular calcium in neurones and cardiac myocytes.