The exposure system [Schonborn et al., 2000] located inside an incubator consisted of two 128.5 x 65 x 424 mm³ brass single-mode waveguide resonators (equipped with DC ventilators) that were assigned to exposure or sham condition by the computer-controlled signal unit ensuring blind conditions for the experiment.
Each resonator was equipped with a plastic holder holding six 35-mm Petri dishes arranged in two stacks in the H-field maxima of the standing wave (E-polarization).
The temperature of the monolayer cells was uniformly distributed without localized "hot spots". The increase in temperature due to the RF EMF was well below 0.1°C per unit SAR. The temperature difference between sham and exposed cells was less than 0.1°C. The absolute uncertainty of the SAR was 20%, and the variation due to the nonuniformity was 29%. The temperature remained at 36.64 ± 0.12°C during the period of measurement, ensuring no thermal effects.
Mess- und Berechnungsdetails
The system was characterized using an FDTD simulation program, and the results were verified by Schuderer et al.  using a DASY3 near-field scanner equipped with dosimetric field and temperature probes.
Schuderer J et al.
High Peak SAR Exposure Unit With Tight Exposure and Environmental Control for In Vitro Experiments at 1800 MHz
Schönborn F et al.
Design, optimization, realization, and analysis of an in vitro system for the exposure of embryonic stem cells at 1.71 GHz.
Lixia S et al.
Effects of 1.8 GHz radiofrequency field on DNA damage and expression of heat shock protein 70 in human lens epithelial cells.
Lee JS et al.
Radiofrequency radiation does not induce stress response in human T-lymphocytes and rat primary astrocytes.
Lantow M et al.
Free radical release and HSP70 expression in two human immune-relevant cell lines after exposure to 1800 MHz radiofrequency radiation.
Chauhan V et al.
Gene Expression Analysis of a Human Lymphoblastoma Cell Line Exposed In Vitro to an Intermittent 1.9 GHz Pulse-Modulated Radiofrequency Field.
Qutob SS et al.
Microarray gene expression profiling of a human glioblastoma cell line exposed in vitro to a 1.9 GHz pulse-modulated radiofrequency field.
Scarfi MR et al.
Exposure to radiofrequency radiation (900 MHz, GSM signal) does not affect micronucleus frequency and cell proliferation in human peripheral blood lymphocytes: an interlaboratory study.
Lim HB et al.
Effect of 900 MHz electromagnetic fields on nonthermal induction of heat-shock proteins in human leukocytes.
Biological stress responses to radio frequency electromagnetic radiation: are mobile phones really so (heat) shocking?
Diem E et al.
Non-thermal DNA breakage by mobile-phone radiation (1800 MHz) in human fibroblasts and in transformed GFSH-R17 rat granulosa cells in vitro.
Nikolova T et al.
Electromagnetic fields affect transcript levels of apoptosis-related genes in embryonic stem cell-derived neural progenitor cells.
Czyz J et al.
High frequency electromagnetic fields (GSM signals) affect gene expression levels in tumor suppressor p53-deficient embryonic stem cells.
Tian F et al.
Exposure to 2.45 GHz electromagnetic fields induces hsp70 at a high SAR of more than 20 W/kg but not at 5W/kg in human glioma MO54 cells.
Leszczynski D et al.
Non-thermal activation of the hsp27/p38MAPK stress pathway by mobile phone radiation in human endothelial cells: Molecular mechanism for cancer- and blood-brain barrier-related effects.
Kwee S et al.
Changes in cellular proteins due to environmental non-ionizing radiation. I. Heat-shock proteins.
Kerbacher JJ et al.
Influence of radiofrequency radiation on chromosome aberrations in CHO cells and its interaction with DNA-damaging agents.
Um diese Webseite für Sie optimal zu gestalten und fortlaufend verbessern zu können, verwenden wir Cookies. Durch die weitere Nutzung der Webseite stimmen Sie der Verwendung von Cookies zu.