Electromagnetic fields affect transcript levels of apoptosis-related genes in embryonic stem cell-derived neural progenitor cells med./bio.

[Elektromagnetische Felder beeinträchtigen Transkript-Levels von Apoptose-verbundenen Genen in neuralen, aus embryonalen Stammzellen gewonnenen Vorläuferzellen]

Ziel der Studie (lt. Redakteur)

Hintergrund/weitere Details

Endpunkt

• verschiedene Wirkungen (z.B. verschiedene Transkript-Gehalte, Genotoxizität, Proliferation, Apoptose, Zytotoxizität, Mitochondrien-Funktion)

Exposition/Befeldung (teilweise nur auf Englisch)

• • 50/60 Hz
• • Hochspannungsfreileitung
  • Mobilfunk
  • GSM

Vollständige Ansicht Kompaktansicht

Methoden Endpunkt/Messparameter/Methodik

• Genotoxizität/Mutation: Einzelstrangbrüche, Doppelstrangbrüche (alkalischer und neutraler Comet-Assay); Chromosomenaberrationen, Schwesterchromatid-Austausch (differenzierte Färbung durch FPG-Technik)
• molekulare Biosynthese: mRNA-Gehalte/Transkript-Level von Zellzyklus-regulierenden (GADD45), Apoptose-verbundenen (bcl-2, bax) und Nerven-spezifischen (Nurr1) Genen (sowie von p53, TH und Nestin; RT-PCR)
• Zellfunktionen: Mitochondrien-Funktion (Mitochondrien-selektive Färbung (Mitotacker CM-H2X ROS))
• Zelllebensfähigkeit/Zellteilung/Zellproliferation: Zellproliferation (BrdU-Inkorporierung); Quantifizierung der Apoptose (Gehalt an hypodiploider DNA; Durchflusszytometrie)

Hauptergebnis der Studie (lt. Autor)

Studie gefördert durch

• European Union (EU)/European Commission
• Fonds der chemischen Industrie (FCI; Foundation of the Chemical Industry), Germany
• VERUM Foundation (Stiftung für Verhalten und Umwelt), Germany

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