Effect of extremely low frequency magnetic fields on cell proliferation and gene expression med./bio.

[Wirkung von extrem niederfrequenten Magnetfeldern auf Zell-Proliferation und Genexpression]

Ziel der Studie (lt. Autor)

Hintergrund/weitere Details

Endpunkt

• molekulare Biosynthese: Genexpression
• Zelllebensfähigkeit/Zellteilung/Zellproliferation: Zellwachstum

Exposition/Befeldung (teilweise nur auf Englisch)

• • 60 Hz
  • 50/60 Hz
  • magnetisches Feld

Vollständige Ansicht Kompaktansicht

Methoden Endpunkt/Messparameter/Methodik

• molekulare Biosynthese: Genexpression (DNA-Microarray und semi-quantitative und quantitative Real-time RT-PCR; nur MCF7 mit 2 mT)
• Zellfunktionen: Zellzyklus-Verteilung (Propidiumiodid-Färbung, Durchflusszytometrie; nur MCF7 mit 2 mT)
• Zelllebensfähigkeit/Zellteilung/Zellproliferation: Zell-Zahl (Trypanblau-Färbung, Hämozytometer; alle Zellen), Zelllebensfähigkeit (MTT-Test, Spektrophotometrie), Zelltod (Propidiumiodid-Färbung, Durchflusszytometrie), Proliferation (Bromdesoxyuridin-Inkorporierung, Immunhistochemie, Spektrophotometrie) (alle Zellen, nur 1 mT), Apoptose (Caspase 3/7-Enzymaktivität, Spektrophotometrie; nur MCF7 mit 2 mT)

Hauptergebnis der Studie (lt. Autor)

Studie gefördert durch

• Inha University, Incheon, Korea
• Korea Institute of Energy Technology and Planning (KETEP), Korea
• National Research Foundation (NRF) of Korea

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• Miyata H et al. (2015): Analysis of gene expression in human umbilical vein endothelial cells exposed to a 50-Hz magnetic field
• Destefanis M et al. (2015): Extremely low frequency electromagnetic fields affect proliferation and mitochondrial activity of human cancer cell lines
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